有机磷杀虫剂多残留免疫分析技术研究进展
有机磷杀虫剂是一类重要的农业生产资料, 在防治害虫和增加粮食产量方面发挥了极大的作用, 但由于其大量使用带来的环境问题越来越受到人们的关注。因此, 如何快速准确地检测其在农作物及环境中的残留、尤其是多种农药的残留, 一直是研究的热点。近年来, 基于抗原抗体特异性识别反应机理, 研究者在农药多残留免疫分析方面做了大量的探索。本文针对有机磷类杀虫剂的多残留免疫分析, 从采用混合抗体、多抗原决定簇抗体、共性结构抗体及单链可变区抗体几个方面做了详细地总结, 并对存在的问题进行了探讨, 以期为今后的研究提供借鉴。
Organophosphorus pesticides (OPs), a kind of important agricultural production material, have been playing an important role in pest control and food production increase. But more and more attention has been paid on the serious environmental problems because of the excess use of OPs. The quick and accurate detection of residues, especially the multi-residues of OPs is always a research focus. In recent years, a great deal of research and exploration has been made on the multi-analyte immunoassay of pesticides based on the spe-cific recognition between antigen and antibody. This article reviewed the recent advances on the mul-ti-residue determination of organophosphorus insecticides, including the progresses of mixed antibodies, antibodies against multi-antigenic determinants, antibodies against common moiety and single chain fragment variable (ScFv) antibodies, and discussed the existed problems and future research.
标题:有机磷杀虫剂多残留免疫分析技术研究进展
英文标题:Research advance on multi-residue immunoassay of organophosphorus insecticides
作者:
祁志军 西北农林科技大学农药研究所
寇立泉 西北农林科技大学农药研究所
王东方 西北农林科技大学农药研究所
刘爽 西北农林科技大学农药研究所
中文关键词:有机磷,多残留,抗原,免疫分析,
英文关键词:organophosphorus,multiresidue,antigen,immunoassay,
发表日期:2013-01-28
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