反相高压液相色谱法测定蜂胶保健食品中白杨素、高良姜素的含量

反相高压液相色谱法测定蜂胶保健食品中白杨素、高良姜素的含量
目的  建立用反相高压液相色谱测定保健食品中白杨素、高良姜素的方法。方法  蜂胶样品中白杨素、高良姜素经甲醇水浴回流提取、C18反相柱分离, 在波长268 nm处检测。结果  此方法得到的白杨素回收率为95.5%~100.8%,  RSD为1.1%~2.0%, 高良姜素回收率为95.2%~100.5%, RSD为1.6%~2.8%。结论  此方法简便快速, 准确可靠, 可用于对一些蜂胶保健食品中白杨素、高良姜素含量的测定。

Objective  To establish a method for the detection of chrysin and galangin in propolis health care foods by reversed-phase high pressure liquid chromatography (RP-HPLC). Methods  Chrysin and galangin in samples were reflux extracted with methanol in water bath, separated by C18 reversed phase column, and detected at wavelength of 268 nm. Results  The recovery of chrysin was 95.6%~100.8% with the relative standard deviations of 1.1%~2.0%, and the recovery of galangin was 95.2%~100.5% with the relative standard deviations of 1.6%~2.8%. Conclusion  The method is simple, rapid, accurate and reliable, which could be used for the detection of chrysin and galangin in propolis health care foods.

标题：反相高压液相色谱法测定蜂胶保健食品中白杨素、高良姜素的含量
英文标题：Determination of chrysin and galangin in propolis health foods by reversed-phase high pressure liquid chromatography

作者：
孙艳芳 黑龙江省疾病控制中心
肖白曼 黑龙江省疾病控制中心
陈彦凤 黑龙江省疾病控制中心
付宇 黑龙江省疾病控制中心
丁笑莉 黑龙江省疾病控制中心

中文关键词:白杨素,高良姜素,反相高压液相色谱法,保健食品,
英文关键词:chrysin,galangin,reversed-phase high pressure liquid chromatography,health care foods,

发表日期：2012-07-09

反相高压液相色谱法测定蜂胶保健食品中白杨素、高良姜素的含量.pdf

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