NDM-1基因新型染料EverGreen实时荧光PCR检测方法的建立

NDM-1基因新型染料EverGreen实时荧光PCR检测方法的建立
研究初步建立了NDM-1基因的实时荧光PCR快速检测鉴定方法。研究根据超级细菌NDM-1序列,设计并合成了检测引物(ND-E-F和ND-E-R),对NDM-1质粒DNA和其他11个标准菌株进行了EVERGREEN实时荧光PCR检测。结果表明,携有NDM-1基因的质粒DNA模板都出现了很强的特异信号,而其他对照病菌均未出现特异荧光信号,证明这套引物及探针具有NDM-1基因检测的特异性。灵敏度测定结果,此体系可检出10fg/μL质粒DNA模板。研究表明,EverGreen实时荧光PCR方法是一种特异、灵敏、快速的NDM-1基因检测方法。

NDM-1 was an important gene of superbacteria with broad-spectrum resistance to antibiotic.A EverGreen realtime PCR method was developed to detect NDM-1gene.Specific primers and EverGreen probe were designed based on the gene sequence in GeneBank.The probe used in this assay were shown to be specific for plasmid with NDM-1 and did not react with other 11 standard bacteria strains. This technique was sensitive with the detection limit of 10 fg/μL DNA.Real-time fluorescent PCR is a specific,sensitive,rapid and highly effective method for the detection and identification of NDM-1.

标题：NDM-1基因新型染料EverGreen实时荧光PCR检测方法的建立
英文标题：The study of detection and identification of NDM-1 by EverGreen realtime PCR

作者：
吴兴海 山东出入境检验检疫局
赵立青 山东出入境检验检疫局
魏晓棠 山东出入境检验检疫局
魏秋月 辽宁出入境检验检疫局技术中心
肖西志 山东出入境检验检疫局
封立平 山东出入境检验检疫局
厉艳 山东出入境检验检疫局

中文关键词:NDM-1,EverGreen实时荧光PCR,检测,,
英文关键词:NDM-1,EverGreen real-time fluorescent PCR,detection,,

发表日期：

NDM-1基因新型染料EverGreen实时荧光PCR检测方法的建立.pdf

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