NDM-1基因TaqMAN实时荧光PCR检测方法的建立

NDM-1基因TaqMAN实时荧光PCR检测方法的建立
研究初步建立了NDM-1基因的实时荧光PCR快速检测鉴定方法。研究根据NDM-1序列,设计并合成了PCR检测引物(ND-T-F和ND-T-R)和探针(ND-T-PROBE),对NDM-1质粒DNA和其他11个标准菌株进行了实时荧光PCR检测。结果表明,携有NDM-1基因的质粒DNA模板都出现了很强的特异信号,而其他对照病菌均未出现特异荧光信号,证明这套引物及探针具有NDM-1基因检测的特异性。灵敏度测定结果,此体系可检出10fg/μL质粒DNA模板。研究表明,TaqMAN实时荧光PCR方法是一种特异、灵敏、快速的NDM-1基因检测方法。

NDM-1 was an important gene of superbacteria with broad-spectrum resistance to antibiotic.A TaqMAN realtime PCR method was developed to detect NDM-1gene.Specific primers and TaqMAN probe were designed based on the gene sequence in GeneBank.The probe used in this assay were shown to be specific for plasmid with NDM-1 and did not react with other 11 standard bacteria strains. This technique was sensitive with the detection limit of 10 fg/μL DNA.Real-time fluorescent PCR is a specific,sensitive,rapid and highly effective method for the detection and identification of NDM-1.

标题：NDM-1基因TaqMAN实时荧光PCR检测方法的建立
英文标题：The study of detection and identification of NDM-1 by TaqMAN realtime PCR

作者：
吴兴海 山东出入境检验检疫局
张云霞 山东出入境检验检疫局
姜英辉 山东出入境检验检疫局
邓明俊 山东出入境检验检疫局
孟怡 山东出入境检验检疫局
雷质文 山东出入境检验检疫局
蒋丹 辽宁出入境检验检疫局技术中心

中文关键词:NDM-1,TaqMAN实时荧光PCR,检测,,
英文关键词:NDM-1,TaqMAN real-time fluorescent PCR,detectio,,

发表日期：

NDM-1基因TaqMAN实时荧光PCR检测方法的建立.pdf

文件大小:

1.21 MB

高速下载