超高效液相色谱-串联质谱法测定鸡组织和 牛组织中的甲苄喹啉和癸氧喹酯

超高效液相色谱-串联质谱法测定鸡组织和 牛组织中的甲苄喹啉和癸氧喹酯
目的 建立超高效液相色谱-串联质谱检测鸡组织和牛组织中甲苄喹啉和癸氧喹酯残留的检测方法。方法 样品采用乙腈提取，亲水-亲脂平衡介质固相萃取柱Hydrophile-Lipophile Balance（HLB） 净化，超高效液相色谱-串联质谱仪检测，外标法定量。结果 甲苄喹啉和癸氧喹酯在0.005~0.250 mg/ kg 范围内线性关系良好，相关系数均大于0.999，方法定量限为0.01 mg/kg，平均回收率范围为77.0%~110%，相对标准偏差范围为1.45%~12.4%。结论 本方法简便、快速、准确、灵敏度高，适用于鸡组织和牛组织中甲苄喹啉和癸氧喹酯残留的检测与确证。

Objective  To develop a method for determination of nequinate and decoquinate residues in chicken tissues and bovine tissues by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods  The residues of nequinate and decoquinate in the test sample were extracted with acetonitrile. The extraction was cleaned with hydrophile-lipophile balance (HLB) cartridge. The residues were determined by UPLC-MS/MS, using external standard method. Results  The limits of quantification were 0.010 mg/kg for nequinate and decoquinate, the standard curves were linear in the range of 0.005~0.250 mg/kg, with the correlation coefficients >0.999. The average recovery was between 77.0%~110%, the RSD was between 1.36%~12.4%. Conclusion  This method is rapid, simple, precise, and sensitive for both confirmation and quantification of nequinate and decoquinate residues in chicken tissues and bovine tissues.

标题：超高效液相色谱-串联质谱法测定鸡组织和 牛组织中的甲苄喹啉和癸氧喹酯
英文标题：Determination of nequinate and decoquinate residues in chicken tissues and bovine tissues by ultra performance liquid chromatography-tandem mass spectrometry

作者：
林黎 深圳出入境检验检疫局食品检验检疫技术中心深圳市食品安全检测技术研发重点实验室
康海宁 深圳出入境检验检疫局食品检验检疫技术中心深圳市食品安全检测技术研发重点实验室
肖陈贵 深圳出入境检验检疫局食品检验检疫技术中心深圳市食品安全检测技术研发重点实验室
沈金灿 深圳出入境检验检疫局食品检验检疫技术中心深圳市食品安全检测技术研发重点实验室
叶刚 深圳出入境检验检疫局食品检验检疫技术中心深圳市食品安全检测技术研发重点实验室

中文关键词:甲苄喹啉,癸氧喹酯,超高效液相色谱-串联质谱法,鸡肉组织,牛肉组织,
英文关键词:nequinate,decoquinate,ultra performance liquid chromatography-mass spectrum spectrometry,chicken tissues,bovine tissues,

发表日期：2014-10-09

超高效液相色谱-串联质谱法测定鸡组织和 牛组织中的甲苄喹啉和癸氧喹酯.pdf

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