清咽类保健食品中非法添加盐酸二氧丙嗪的 检测方法研究

清咽类保健食品中非法添加盐酸二氧丙嗪的 检测方法研究
目的　建立对保健食品中非法添加的盐酸二氧丙嗪的筛查方法。方法　采用薄层色谱法(TLC)进行快速筛查, 采用高效液相色谱法(HPLC)对盐酸二氧丙嗪进行定性分析以及定量测定, 并采用超高效液相-质谱联用法(UPLC-MS)进行确证。结果　薄层色谱法盐酸二氧丙嗪斑点显色明显; 高效液相色谱法在11.149?222.977 μg/mL范围内线性良好, 质谱图中盐酸二氧丙嗪分子离子峰及二级碎片离子明显。 结论　本方法专属性强, 灵敏度高, 重现性好, 可作为清咽类保健食品是否添加盐酸二氧丙嗪的检测方法。

Objective  To establish a method for identification of dioxopromethazine hydrochloride illegally added in health care food. Methods  The dioxopromethazine hydrochloride was identified by thin layer chromatography (TLC) and ultra performance liquid chromatography quadrupole time of flight mass spectrometry(UPLC-QTOF-MS), and the content was determined by high performance liquid chro-matography (HPLC). Results  The linear range of the method was between 11.149 and 222.977 μg/mL, and the regression equation was Y=4.8870×104X+8.6351×103 with correlation coefficient of 0.9999. The detection limit was 1.115 μg/mL. Conclusion  The method is convenient, reliable and accurate. It can be used to detect illegal adulteration of dioxopromethazine hydrochloride in health care food.

标题：清咽类保健食品中非法添加盐酸二氧丙嗪的 检测方法研究
英文标题：Determination of dioxopromethazine hydrochloride illegally adulteration in health care food

作者：
吴景 中国食品药品检定研究院
陈骁鹏 泰州市食品药品检验所
袁华峰 泰州市食品药品检验所
曹进 中国食品药品检定研究院
钱忠义 泰州市食品药品检验所
邢书霞 中国食品药品检定研究院

中文关键词:保健食品,盐酸二氧丙嗪,非法添加,高效液相色谱法,超高效液相-飞行时间质谱联用法,
英文关键词:health food,dioxopromethazine hydrochloride,illegally adulteration,high performance liquid chromatography,ultra performance liquid chromatography-quadrupole time of flight mass spectrometry,

发表日期：2014-07-24

清咽类保健食品中非法添加盐酸二氧丙嗪的 检测方法研究.pdf

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