沙棘果渣总黄酮提取工艺响应面法优化与 抗氧化活性研究

沙棘果渣总黄酮提取工艺响应面法优化与 抗氧化活性研究
目的  优选沙棘果渣总黄酮提取的最佳工艺, 并对其抗氧化活性进行评价。方法  选取提取时间、温度、料液比、乙醇体积分数为影响因素, 在单因素实验结果基础上确定各因素的分析水平, 利用响应面中心组合法设计实验方案。并通过体外抗氧化能力指数(ORAC)法研究沙棘果渣总黄酮的抗氧化能力。结果  建立了沙棘果渣总黄酮提取的数学模型, 最佳提取工艺条件为乙醇体积分数70%、提取温度 80℃、提取时间100 min、液料比25:1, 沙棘果渣总黄酮具有较强抗氧化活性, 与浓度成正相关。结论  本研究结果可为沙棘果渣总黄酮工业化提取和应用提供理论指导。

Objective  To explore the optimal extraction conditions of total flavonoids from residue of Hippophae rhamnoides L. and evaluate the antioxidant activity. Methods  Based on the single-factor tests, the conditions of total flavonoids from residue of Seabuckthorn were optimized by response surface metho-dology in order to increase the extraction yield of total flavonoids. Meanwhile, the antioxidant activity of total flavonoids was assessed by oxygen radical absorbent capacity (ORAC). Results  The optimal extrac-tion conditions of total flavonoids were ethanol concentration 70%, material-liquid ratio 1:25, extraction time 100 min and extraction temperature 80℃. Under the optimal conditions, the maximum extraction yield of total flavonoids was 2.73%. Extracted total flavonoids had a strong antioxidant activity in a dose-dependent manner. Conclusion  These investigations will be reference a certain theoretical instruction and application value for the extraction of flavonoids in Seabuckthorn.

标题：沙棘果渣总黄酮提取工艺响应面法优化与 抗氧化活性研究
英文标题：Optimizaton of extraction of total flavones from residue of Hippophae rhamnoides L. using response surface methodology and their antioxidant activity

作者：
田景民 内蒙古大学生命科学学院;内蒙古医科大学药学院
陈贵林 内蒙古大学生命科学学院;内蒙古自治区中蒙药材规范化生产工程技术研究中心

中文关键词:沙棘,黄酮,提取,响应面,抗氧化,
英文关键词:Hippophae rhamnoides L.,flavonoids,extraction,response surface methodology,antioxidant activity,

发表日期：2014-07-03

2025-1-28 18:49 上传 沙棘果渣总黄酮提取工艺响应面法优化与 抗氧化活性研究.pdf

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