无需衍生化样品处理快速测定食品中 黄曲霉毒素B1
目的 建立无需衍生化法-超高效液相色谱直接测定食品中的黄曲霉毒素B1的快速检测方法。 方法 样品经甲醇-水(7:3, v:v)提取, 免疫亲和柱净化, C18色谱柱分离后, 采用大体积流通池荧光检测器检测。结果 黄曲霉毒素B1在0.10~5.00 ng/mL范围内与峰面积呈良好线性关系, R2>0.999, 在饼干、粉丝、点心、沙琪玛、蛋糕、辣椒酱6种基质中, 黄曲霉毒素B1在0.50~2.00 μg/kg范围内加标, 平均回收率在71.7%~111.2%之间, 相对标准偏差(RSDs)为3.8%~11.5%, 检出限(LOD)为0.05 μg/kg。结论 本方法灵敏、快速、无衍生、结果准确, 能够满足食品中黄曲霉毒素B1残留的检测需求。
Objective To establish a rapid determination of aflatoxin B1 in food by ultra performance liquid chromatography (UPLC) without derivation method. Methods Samples were extracted with methanol water (7:3, v:v), cleaned up by immuno-affinity column, separated by C18 column, and determined by fluorescence detector with large volume flow cell. Results High correlation coefficient (r2>0.999) was obtained in the linear range of 0.10~5.00 ng/mL. The average recovery rates were 71.7%~111.2% at the spiked level of 0.50~2.00 μg/kg and relative standard deviation(RSD) was 3.8%~11.5% in biscuit, vermicelli, refreshment, sacima, cakes, and chili sauce. The limit of quantitation (LOD) was 0.05 μg/kg. Conclusion This method is sensitive, rapid, non-derived, and accurate, and it can meet the requirements for determination of aflatoxin B1 in foods.
标题：无需衍生化样品处理快速测定食品中 黄曲霉毒素B1
英文标题：Rapid determination of aflatoxin B1 in foods without derivation
作者：
雷红琴 伊犁出入境检验检疫局综合技术服务中心
李芳 伊犁出入境检验检疫局综合技术服务中心
黎琳琳 伊犁出入境检验检疫局综合技术服务中心
刘绪斌 伊犁出入境检验检疫局综合技术服务中心
沙力 伊犁出入境检验检疫局综合技术服务中心
李艳美 伊犁出入境检验检疫局综合技术服务中心
储晓刚 中国检验检疫科学研究院
粟有志 伊犁出入境检验检疫局综合技术服务中心
中文关键词:黄曲霉毒素B1,超高效液相色谱法,免疫亲和柱,无衍生,
英文关键词:aflatoxin B1,ultra performance liquid chromatography,immuno-affinity column,without derivation,
发表日期：2014-07-22
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