Objective To establish a mathematical model of uncertainty evaluation of deoxytetracycline in liquid milk by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method. Methods The sources of uncertainty of the whole determination procedure were analyzed and the combined uncertainty was finally obtained. Results The expanded uncertainty of 0.0056 μg/g (k=2) with 0.052 μg/g of the content of deoxytetracycline was derived. The major sources of uncertainty of measurement include the repeatability of detection and the curve fitting. Conclusion It is necessary to control experimental conditions and the linear of standard curve to make sure the testing results are accurate.
标题：超高效液相色谱-串联质谱法测定牛奶中强力霉素残留的不确定度评估
英文标题：Uncertainty evaluation of deoxytetracycline in liquid milk by ultra performance liquid chromatography-tandem mass spectrometry
