Researchonratmodelsofhypobarichypoxiainducedpulmonaryhypertension.pdf
OBJECTIVE:Ratmodelsofhypobarichypoxia-inducedpulmonaryhypertensionarecommonlyusedinstudiesofchronicmountainsickness,whiletherearefewresearchesspeciallyfocusingontheseratsmodel.Thisstudyaimstoexploringpossiblepathogenesisofhypobarichypoxia-inducedpulmonaryhypertensionbyexperimentingonhypobarichypoxia-inducedPHratmodelsatdifferentsimulate-altitudes.MATERIALSANDMETHODS:32healthymaleSDratswererandomlydividedintosixgroupsofdifferentdegreeandtimeperiodofhypobarichypoxia.Themeanpulmonaryarterialpressure(mPAP),rightventricularpressure(RVSP),therightventricle(RV),leftventricular(LV),ventricularseptal(S),therightventricularhypertrophyindex(RVHI)[calculatedundertheformulaofRV/(LV+S)],hematoxylin-eosinstaining,elasticfibersstaining,theratioofthethicknessofvascularwalltoitsouterdiameter(MT%),theratioofthecross-sectionalareaofthemiddlevascularwalltothetotalvascularcross-sectionalarea(MA%);theα-SMA,andtheKi6expressionsweredetectedtoevaluatedthepulmonaryhypertension.RESULTS:ThereweresignificantdifferencesofthemPAP,RVSPandRVHIvaluebetweenthehypobarichypoxiagroupsandthecontrolgroup(p<0.05).ThemPAP,RVSP,RVHI,MT%,MA%,α-SMA,andKi6ofratsinmodelgroupsatanaltitudeof3KMwerehigherthanthoseofthecontrolgroup,whichraisedgraduallywiththenumberofweeksincreasing.ThemPAP,RVSP,RV/(LV+S)value,MT%,MA%,α-SMA,andKi67ofthe5KM-4Wgroupweresignificantlyhigherthanthoseofthecontrolgroup(p<0.05).CONCLUSIONS:Ratmodelswithpulmonaryhypertensionatdifferentaltitudeshavebeensuccessfullyestablishedbyautomaticadjustinghypobarichypoxiachamber.Exposuretoalowoxygenenvironmentatasimulate-altitudeof3kmfor8weekshavecausedthepathologicalremodelingofpulmonaryvascularwallsandpulmonaryhypertension,andfurtherledtoaseriesofpathologicalchanges,includingrightventricularhypertrophy.Thismodeliseasytoberepli-catedwithgoodreproducibilityandprovidesevidenceforclinicaltrialofdrugs.
作者：T.-T.MA Y.WANG X.-L.ZHOU H.JIANG R.GUO L.-N.JIA H.CHANG Y.GAO X.-Y.YAO Z.-M.GAO L.PAN
作者单位：DepartmentofGeriatricMedicine,BeijingShijitanHospital,CapitalMedicalUniversity,Beijing,China;DepartmentofHypoxiaLaboratory,BeijingShijitanHospital,CapitalMedicalUniversity,Beijing,ChinaDepartmentofPathology,BeijingShijitanHospital,CapitalMedicalUniversity,Beijing,ChinaDepartmentofHypoxiaLaboratory,BeijingShijitanHospital,CapitalMedicalUniversity,Beijing,China;HealthScienceCenterofPekingUniversity,Beijing,ChinaDepartmentofHypoxiaLaboratory,BeijingShijitanHospital,CapitalMedicalUniversity,Beijing,China
母体文献：青藏铁路运营十周年学术研讨会论文集
会议名称：青藏铁路运营十周年学术研讨会
会议时间：2016年6月26日
会议地点：拉萨
主办单位：中国铁道学会
语种：chi
分类号：
关键词：Hypobarichypoxia Pulmonaryhypertension Animalmodels
在线出版日期：2019年4月17日
基金项目：
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