微波水解-茚三酮柱后衍生离子交换色谱法测定鹌鹑蛋中的17种氨基酸
微波水解-茚三酮柱后衍生离子交换色谱法测定鹌鹑蛋中的17种氨基酸
目的建立微波水解茚三酮柱后衍生离子交换色谱法同时测定鹌鹑蛋中17种氨基酸含量的分析方法。方法采用磺酸型阳离子树脂离子交换柱分离, 茚三酮溶液作为柱后衍生剂, 紫外可见分光光度检测器进行检测, 检测波长为570 nm和440 nm, 并对微波水解时间及温度进行条件优化。结果17种氨基酸在一定范围内呈良好线性关系, 相关系数均大于0.99; 检出限为0.376~0.906 mg/L。结果表明, 150 ℃下微波水解 20 min的结果与传统加热水解(110 ℃, 22 h)的效果基本相同。结论该方法分析时间较短, 灵敏度较高, 可用于鹌鹑蛋中氨基酸含量的检测。
ObjectiveTo establish a method for simultaneous determination of 17 amino acids in quail eggs by microwave-hydrolyzed ninhydrin column post-derivative ion exchange chromatography. MethodsThe sulfonium-type cationic resin ion exchange column was used for separation, and the ninhydrin solution was used as a post-column derivatizer. The UV-visible spectrophotometer was used for detection. The detection wavelengths were 570 nm and 440 nm, and the microwave hydrolysis time and temperature were optimized. ResultsThe 17 amino acids showed a good linear relationship in the range of 0.005?1.000 μmol/mL, and the correlation coefficient was greater than 0.99. The limits of detection were 0.376?0.906 mg/L. The results of microwave hydrolysis at 150 ℃ for 20 min were basically the same as those of traditional heating hydrolysis (110 ℃, 22 h). ConclusionThis method has short analysis time and high sensitivity, and can be used for detecting the amino acid content in quail eggs.
标题：微波水解-茚三酮柱后衍生离子交换色谱法测定鹌鹑蛋中的17种氨基酸
英文标题：Determination of 17 kinds of amino acids in quail eggs by microwave hydrolysis-ninhydrin column post-derivative ion exchange chromatography
作者：
柯丽群 东莞市中鼎检测技术有限公司
王同珍 东莞市中鼎检测技术有限公司
曹维强 东莞市中鼎检测技术有限公司
吴奕煌 东莞市中鼎检测技术有限公司
詹衬开 东莞市中鼎检测技术有限公司
郑宇丽 东莞市中鼎检测技术有限公司
中文关键词:微波水解,氨基酸,茚三酮柱后衍生离子交换色谱法,
英文关键词:microwave hydrolysis,amino acid,ninhydrin column post-derivative ion exchange chromatography,
发表日期：2019-06-27
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