超高效液相色谱-串联质谱法同时测定鸡肉中43种兽药残留
目的 建立正负离子切换-超高效液相色谱-串联质谱法同时测定鸡肉中43种兽药残留量的分析方法。方法 鸡肉样品经甲酸提取液涡旋提取, 固相萃取净化, 10 mmol/L甲酸铵稀释, ACQUITY UPLC HSS T3色谱柱(2.1 mm×100 mm, 1.8 μm)色谱柱分离, 以0.05%甲酸溶液和0.05%乙腈进行梯度洗脱, 正离子多反应监测39种兽药, 负离子多反应检测4种兽药, 外标法定量测定药物的含量。结果 43种兽药定量限为5 μg/kg, 标准曲线在0.4~100.0 ng/mL范围内均呈良好的线性关系。在空白鸡肉中3个不同添加水平下, 平均回收率为60%~120%之间, 变异系数为0.7%~12%。结论 该方法准确、快速、灵敏、稳定, 适合作为鸡肉中43种兽药残留量的测定。
Objective To establish a method for simultaneous determination of 43 kinds of veterinary drug residues in chicken by ion switching-ultra performance liquid chromatography tandem mass spectrometry. Methods Chicken samples were extracted by eddy extraction of formic acid extract, purified by solid phase extraction, diluted by 10 mmol/L ammonium formate, and separated by ACQUITY UPLC HSS T3 column (2.1 mm×100 mm, 1.8 μm), gradient elution with 0.05% formic acid solution and 0.05% acetonitrile. Thirty-nine kinds of veterinary drugs were monitored by positive ion multi-reaction, 4 kinds of veterinary drugs were monitored by negative ion multi-reaction, and all the 43 kinds of drug residues were quantitatively determined by external standard method. Results The limits of quantitative of 43 veterinary drugs were 5 μg/kg, and the standard curve showed a good linear relationship in the range of 0.4-100.0 ng/mL. The recoveries at 3 different levels in the blank chicken were 60%-120%, with the variable coefficient of 0.7%-12%. Conclusion This method is accurate, rapid, sensitive and stable, which is suitable for the determination of 43 kinds of veterinary drug residues in chicken.
标题：超高效液相色谱-串联质谱法同时测定鸡肉中43种兽药残留
英文标题：Determination of 43 kinds of veterinary drug residues in chicken meat by ultra performance liquid chromatography tandem mass spectrometry
作者：
金晓峰 贵州省兽药饲料监察所
焦仁刚 贵州省兽药饲料监察所
赵贵 贵州省兽药饲料监察所
罗永成 贵州省兽药饲料监察所
黄鑫 贵州省兽药饲料监察所
栾庆祥 贵州省兽药饲料监察所
周艺林 贵州省兽药饲料监察所
郭萍 贵州省兽药饲料监察所
周泽晓 贵州省兽药饲料监察所
廖欢 贵州省兽药饲料监察所
中文关键词:正负离子切换,超高效液相色谱-串联质谱法,鸡肉,同时测定,兽药残留,
英文关键词:ion switching,ultra performance liquid chromatography-tandem mass spectrometry,chicken,simultaneous determination,veterinary drug residues,
发表日期：2019-05-21
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