LC-MSMS法测定保健食品中左卡尼汀的含量
目的 建立保健品中左卡尼汀的LC-MS/MS的测定方法。方法 待测样品经超声提取、稀释后, 分别采用CAPCELL PAK CR色谱柱和PC HILIC色谱柱进行分离, 最后用LC-MS/MS进行检测。 结果 使用CAPCELL PAK CR法检测左卡尼汀能够得到更高的灵敏度, 线性范围为0~200 ng/mL, 相关系数为r=0.9995。结论 该方法灵敏度高、准确、重现性好, 可以用于一些保健食品中左卡尼汀的含量检测。
Objective To establish a method for the determination of levocarnitine in health care food by LC-MS/MS. Methods After an ultrasonic extraction and dilution, the samples were separated by CAPCELL PAK CR and PC HILIC column, respectively. Finally the target compound was detected by LC-MS/MS. Results Compared with the PC HILIC column, using CAPCELL PAK CR column could get a higher sensitivity result. The method showed a good linearity over the range of 0~200.0 ng/mL for levocarnitine with r=0.9995. Conclusion This method has a good sensitivity, accuracy and reproducibility and it is suitable for the detection of levocarnitine in health care food.
标题：LC-MSMS法测定保健食品中左卡尼汀的含量
英文标题：Determination of levocarnitine in health care food by LC-MS/MS
