气相色谱法测定蔬菜中敌百虫的含量
气相色谱法测定蔬菜中敌百虫的含量
目的建立气相色谱法测定蔬菜中敌百虫的含量。方法研究不同色谱条件及消除基质效应对敌百虫测定的影响, 并取蔬菜样品(以青瓜为代表)10 g进行前处理提取后, 用经优化后的色谱条件对敌百虫进行测定, 外标法定量。结果在进样口温度设为150 ℃, 使用内径0.32 mm的色谱柱以及设置3.0 mL/min的色谱柱流量时, 敌百虫响应较好; 在0.06~2.0 mg/kg的加标水平下, 敌百虫回收率在90.2%~99.8%之间, 相对标准偏差≤4.3%。结论通过对敌百虫气相色谱条件的优化以及使用基质标准曲线消除基质效应, 可以大幅提高气相色谱法检测敌百虫的准确度。
ObjectiveTo establish the gas chromatography method to determine the content of trichlorfon in vegetables. MethodsThe influence of different chromatographic conditions and elimination of matrix effect on the determination of trichlorfon was studied. After taking 10 g of vegetable samples (represented by cucumber) as pretreatment and extraction, the trichlorfon were determined by optimized chromatographic conditions, and quantified by the external standard method. ResultsWhen the injector temperature was set to 150 ℃, a chromatographic column with an inner diameter of 0.32 mm was used and a flow rate of 3.0 mL/min was set, the response of trichlorfon was better. At the standard level of 0.06–2.0 mg/kg, the recovery rate of trichlorfon ranged from 90.2% to 99.8%, with the relative standard deviation ≤4.3%. ConclusionBy optimizing the gas chromatography conditions of trichlorfon and using matrix standard curves to eliminate matrix effects, the accuracy of gas chromatography detection of trichlorfon can be greatly improved.
标题：气相色谱法测定蔬菜中敌百虫的含量
英文标题：Determination of trichlorfon in vegetables by gas chromatography
作者：
黄航星 广东省食品工业公共实验室；广东省食品工业研究所有限公司；广东省食品质量监督检验站
陈燕敏 广东省食品工业公共实验室；广东省食品工业研究所有限公司；广东省食品质量监督检验站
郭海柔 广东省食品工业公共实验室；广东省食品工业研究所有限公司；广东省食品质量监督检验站
何焜鹏 广东省食品工业公共实验室；广东省食品工业研究所有限公司；广东省食品质量监督检验站
中文关键词:敌百虫,温度,热分解,基质效应,
英文关键词:trichlorfon,temperature,thermal decomposition,matrix effect,
发表日期：2020-05-13
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