超高效液相色谱-串联质谱法检测植物源加工食品中农药残留
超高效液相色谱-串联质谱法检测植物源加工食品中农药残留
目的建立超高效液相色谱-串联质谱法检测植物源加工食品中35种农药残留的方法。方法样品经乙腈提取, QuEChERS方法净化, 用Shim-pack GIST C18色谱柱分离, 以乙腈和0.1％甲酸水溶液为流动相进行梯度洗脱, 电喷雾正离子/负离子模式电离, 多反应监测扫描模式检测, 外标法定量。结果35种化合物在0.002~0.2 μg/mL范围内均呈现良好的线性关系, 相关系数(r)均大于0.990。检出限为0.04~3.43 μg/kg, 苹果泥样品中平均加标回收率为62%~97%, 相对标准偏差为2.4%～9.1%。结论本方法重现性好, 回收率高, 可实现植物源加工食品中35种农药残留的同时定性、定量分析。
ObjectiveTo establish a method for the determination of 35 pesticide residues in processed food of plant origin by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). MethodsThe samples were extracted with acetonitrile, purified by QuEChERS method, and separated with Shim-pack GIST C18 chromatographic column for gradient elution using acetonitrile and 0.1% formic acid aqueous solution as mobile phase, ionized by electrospray positive/negative ion mode, detected by multiple reaction monitoring scanning mode and quantified by external standard method. ResultsA total of 35 kinds of compounds had good linear relationships in the range of 0.002?0.2 μg/mL, and the correlation coefficients were larger than 0.990. The limits of detection were 0.04?3.43 μg/kg. The average standard recoveries of apple puree were 62%?97% and the relative standard deviations were 2.4%?9.1%. ConclusionThis method has good reproducibility and high recovery rates. It can realize the simultaneous qualitative and quantitative analysis of 35 pesticide residues in processed food of plant origin
标题：超高效液相色谱-串联质谱法检测植物源加工食品中农药残留
英文标题：Determination of pesticide residues in processed foods of plant originby ultra performance liquid chromatography tandemmass spectrometry
作者：
刘瑜 沈阳海关
张彤 沈阳海关
杨潇 沈阳海关
李晓东 沈阳海关
姜玲玲 沈阳海关
金雁 沈阳海关
姜莉 沈阳海关
中文关键词:植物源加工食品,农药残留,超高效液相色谱-串联质谱法,
英文关键词:processed food of plant origin,pesticide residues,ultra performance liquid chromatography tandem mass spectrometry,
发表日期：2020-08-18
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