能力验证中单增李斯特菌的分离与鉴定
能力验证中单增李斯特菌的分离与鉴定
目的通过参加CNAS比对能力验证计划, 提高实验室检测校准能力。方法依据GB 4789.30-2016《食品安全国家标准 食品微生物学检验 单核细胞增生李斯特氏菌检验》的基础方法和生化分子学方法相结合, 对盲样进行定性检测。结果2个盲样LMO(ZJ)-191和LMO(ZJ)-134分别为检出和未检出单增李斯特菌, 检测结果为均通过。结论本次采用多种组合实验方法, 更加准确了解到单增李斯特氏菌的各项特征, 综合提升了检测准确度和效率。
ObjectiveTo improve the laboratory detection and calibration ability by participating in the CNAS comparison ability verification plan. MethodsAccording to the basic method of GB 4789.30?2016 "National food safety standards-Food microbiological analysis-Detection of Listeria monocytogenes" and biochemical molecular methods, the blind samples were qualitatively detected. ResultsLMO(ZJ)-191 was identified as Listeria monocytogenes while Listeria monocytogenes was not detected in LMO(ZJ)-134. The test results were all passed. ConclusionIn this study, a variety of combined experimental methods are used to more accurately understand the characteristics of Listeria monocytogenes, and it comprehensively improves the detection accuracy and efficiency.
标题：能力验证中单增李斯特菌的分离与鉴定
英文标题：Isolation and identification of Listeria monocytogenes in CNASproficiency testing
作者：
邓晓鸿 镇江市食品药品监督检验中心
强敏 镇江市产品质量监督检验中心
唐刚 蚌埠产品质量监督检验研究院
严飞 镇江市妇幼保健院新生儿科
陆源 镇江市食品药品监督检验中心
唐双双 镇江市食品药品监督检验中心
曹伟 镇江市食品药品监督检验中心
中文关键词:实验室,能力验证,单增李斯特菌,
英文关键词:laboratory,proficiency testing,Listeria monocytogenes,
发表日期：2020-06-16
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