QuEChERS结合气相色谱-质谱联用法检测蔬菜中10种农药残留
QuEChERS结合气相色谱-质谱联用法检测蔬菜中10种农药残留
目的建立QuEChERS结合气相色谱-质谱联用法(gas chromatography-mass spectrometry, GC-MS)同时测定蔬菜中10种农药残留的分析方法。方法样品加入乙腈后使用漩涡混合和超声波提取, 应用 QuEChERS 方法进行净化处理。浓缩定容后用GC-MS在选择离子监测模式(select ion monitoring, SIM)下进行测定, 外标法定量。结果10种农药在0.005~1.0 μg/mL范围内线性良好, 相关系数r2均大于0.997, 方法的检出限为0.0001~0.002 mg/kg, 方法的定量限为0.0001~0.007 mg/kg。在0.02、0.05和0.1 mg/kg添加水平的回收率为62.6%~151.2%, 相对标准偏差为0.1%~6.0% (n=6)。结论该方法简单、准确、灵敏高, 适合用于蔬菜中农药多残留的测定。
ObjectiveTo establish a method for the simultaneous determination of 10 pesticide residues in vegetables by QuEChERS combined with gas chromatography-mass spectrometry(GC-MS). MethodsThe samples were extracted by vortex mixing and ultrasonic wave after adding acetonitrile, and purified by the modified QuEChERS method. After concentration and dissolution, the compounds were analyzed by GC-MS under selected ion monitoring (SIM) mode. The external standard method was used for quantitative analysis. ResultsThe 10 pesticides had good linear relationships in the range of 0.005–1.0 μg/mL, and the correlation coefficients were larger than 0.997. The detection limits of the method were 0.0001-0.002 mg/kg, the limits of quantitation were 0.0001–0.007 mg/kg. The recoveries of 10 pesticides at 0.02, 0.05 and 0.1 mg/kg levels ranged from 62.6%–151.2%, and the relative standard deviation were 0.1%–6.0% (n=6). ConclusionThis method is simple, accurate and sensitive, which is suitable for detecting 10 pesticide residues in vegetables.
标题：QuEChERS结合气相色谱-质谱联用法检测蔬菜中10种农药残留
英文标题：Determination of 10 kinds of pesticides residues in vegetables by QuEChERS coupled with gas chromatography-mass spectrometry
作者：
罗欣阳 广东省食品检验所
郑悦珊 广东省食品检验所
熊含鸿 广东省食品检验所
王立亚 广东省食品检验所
梁旭霞 广东省食品检验所
中文关键词:QuEChERS,气相色谱-质谱联用法,蔬菜,农药残留,
英文关键词:QuEChERS,gas chromatography-mass spectrometry,vegetables,pesticide residues,
发表日期：2020-05-27
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