Real-time环介导等温扩增检测枣花蜜源成分
Real-time环介导等温扩增检测枣花蜜源成分
目的建立real-time环介导等温扩增技术(loop-mediated isothermal amplification, LAMP)检测枣花蜜源成分的方法。方法以枣转录间隔区(internal transcribed spacer, ITS)为靶序列, 用软件Primer Explorer V5与软件Oligo 7设计LAMP特异性引物, 优化反应温度, 测试灵敏度、特异性和适用性。结果所建立的方法在反应温度55 ℃条件下, 引物特异性强, 最低检测限为≥1 pg/μL大枣DNA。结论该方法具有特异性强、灵敏度高、操作简单等优势, 适合商业枣花蜜掺假快速检测。
ObjectiveTo establish a real-time loop-mediated isothermal amplification (LAMP) method for the detection of Fructus jujubae nectar. MethodsThe LAMP-specific primers were designed with Primer Explorer V5 and Oligo 7 to optimize the reaction temperature, sensitivity, specificity and applicability using the internal transcribed spacer (ITS) as the target sequence. ResultsThe established method was of high specificity under the condition of reaction temperature of 55 ℃, and the detection limit was≥1 pg/μL genomic DNA of jujube. ConclusionThe method has the advantages of high specificity, high sensitivity and simple operation, which is suitable for rapid detection of jujube nectar adulteration in commercial.
标题：Real-time环介导等温扩增检测枣花蜜源成分
英文标题：Detection of nectar-derived components from jujube flower by real-time loop mediated isothermal amplification
作者：
张萌 河南科技大学食品与生物工程学院；许昌学院河南省食品安全生物标识快检技术重点实验室
古绍彬 河南科技大学食品与生物工程学院
于江晗 河南科技大学食品与生物工程学院
王德国 许昌学院河南省食品安全生物标识快检技术重点实验室
中文关键词:枣花蜜,掺假,转录间隔区,环介导等温扩增,
英文关键词:jujube nectar,adulteration,internal transcribed spacer,loop-mediated isothermal amplification,
发表日期：2020-02-27
页:
[1]