超高效液相色谱串联质谱法检测奶粉中苯甲酸的含量
超高效液相色谱串联质谱法检测奶粉中苯甲酸的含量
目的建立一种超高效液相色谱-串联质谱(ultra performance liquid chromatography-tandem mass spectrometry, UPLC-MS/MS)检测奶粉中苯甲酸(benzoic acid)含量的分析方法。方法样品经水提取后, 选用Waters Acquity UPLC BEH C18色谱柱(2.1 mm×50 mm, 1.7 μm), 以甲醇和0.1%氨水作为流动相进行梯度洗脱, 电喷雾离子化(electrospray ionizatio, ESI-)及多反应监测模式(multi-reaction monitoring, MRM)进行测定, 外标法定量。结果苯甲酸在0.04~2.00 μg/mL浓度范围内, 线性关系良好(r2=0.9996), 样品加标回收率为94.92%~109.31%, 定量限为0.06 μg/g, 结果的相对标准偏差为1.69%~6.90%。结论该方法前处理简单, 稳定性和可靠性好, 具有良好的定性和定量分析能力, 适用于奶粉中苯甲酸含量的检测。
ObjectiveTo establish a method for the determination of benzoic acid in milk powder by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). MethodsAfter the sample was extracted by water, it was separated with a Waters Acquity UPLC BEH C18 column (2.1 mm×50 mm, 1.7 μm) by gradient elution using methanol and 0.1% ammonia as mobile phase, performed by electrospray ionization (ESI-) and multi-reaction monitoring (MRM), and quantified by external standard method. ResultsBenzoic acid had a good linear relationship in the concentration range of 0.04?2.00 mug/mL (r2=0.9996). The recoveries of the spiked samples were 94.92%?109.31%, the limit of quantification was 0.06 μg/g, and the relative standard deviations of the results were 1.69%?6.90%. ConclusionThis method has simple pretreatment, good stability and reliability, good qualitative and quantitative analysis ability, and is suitable for detecting benzoic acid content in milk powder.
标题：超高效液相色谱串联质谱法检测奶粉中苯甲酸的含量
英文标题：Determination of benzoic acid in milk powder by ultra performance liquid chromatography-tandem mass spectrometry
作者：
丁洋 圣元营养食品有限公司
姜勇 圣元营养食品有限公司
纪赟 圣元营养食品有限公司
丁岚 圣元营养食品有限公司
王锡青 圣元营养食品有限公司
中文关键词:苯甲酸,超高效液相色谱串联质谱法,奶粉,
英文关键词:benzoic acid,ultra performance liquid chromatography-tandem mass spectrometry,milk powder,
发表日期：2019-09-29
页:
[1]