液相色谱法测定油脂及其制品中苯并a芘含量
液相色谱法测定油脂及其制品中苯并a芘含量
目的建立液相色谱法测定油脂及其制品中苯并(a)芘的方法。方法试样经过正己烷提取, 中性氧化铝或分子印迹小柱净化, C18色谱柱分离, 荧光检测器检测。结果方法的线性范围为0.5~20 μg/L, 线性相关系数为0.9999, 最低定量浓度(the limit of quantity, LOQ)为0.5 μg/kg, 在0.5、5、15 μg/kg 3个添加水平下平均回收率为75.5%~93.8%, 相对标准偏差(relative standard deviation, RSD)为3.0%~9.6%。结论该方法准确度与精密度满足GB/T 27404规定, 定量限满足GB 2762实施要求, 被用于修订GB/T 5009.27-2003为 GB 5009.27-2016。
ObjectiveTo establish a method for determination of benzo(a)pyrene in fats and oils and their products samples by liquid chromatography (LC). MethodsThe samples were extracted by N-hexane, purified by neutral alumina or molecularly imprinted column, separated by C18 column and detected by fluorescence detector. ResultsThe method showed a good linearity in the range of 0.5?20 μg/L with correlation coefficients of 0.9999, and the limit of quantitation (LOQ) was 0.5 μg/kg. The average recoveries were 75.5%?93.8% at the spiked level of 0.5, 5, 15 μg/kg with the relative standard deviation (RSD) of 3.0%?9.6%. ConclusionThe accuracy and precision of this method meet the requirements of GB/T 27404. The limit of quantitation meets the implementation requirements of GB 2762. This method has been used to revise GB/T 5009.27-2003 to GB 5009.27-2016.
标题：液相色谱法测定油脂及其制品中苯并(a)芘含量
英文标题：Determination of benzo(a)pyrene in fats and oils and their products by liquid chromatography
作者：
滕小沛 江苏省疾病预防控制中心
阮丽萍 江苏省疾病预防控制中心
刘华良 江苏省疾病预防控制中心
中文关键词:液相色谱法,油脂及其制品,苯并(a)芘,
英文关键词:liquid chromatography,fats and oils and their products,benzo(a)pyrene,fats,oils,
发表日期：2019-09-11
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