苎麻籽总黄酮含量测定方法比较研究
苎麻籽总黄酮含量测定方法比较研究
目的比较苎麻籽总黄酮含量的测定方法。方法以芦丁和槲皮素为对照品, 比较直接测定法、NaNO2-Al3+-NaOH法、AlCl3法3种比色方法下苎麻籽(70%乙醇提取液)的紫外-可见光谱图, 并通过精密度、稳定性、干扰试验、加标回收率试验对所选方法进行评价。结果芦丁和槲皮素均不适合作为直接测定苎麻籽总黄酮的对照品; NaNO2-Al3+-NaOH法最大吸收波长为500 nm, 但受某些非黄酮类物质干扰, 使得该方法下的总黄酮含量测定值虚高; AlCl3法以芦丁为对照品, 测定波长为401 nm, 在0.004~0.02 mg/mL范围内浓度与吸光度值成良好的线性关系, 相关系数r2=0.9998, 该法受干扰小, 精密度高, 平均回收率为(99.46±4.16)%, 显色后在20~60 min内吸光值稳定。结论AlCl3法简单可行, 适用于苎麻籽提取物中总黄酮含量的测定。
ObjectiveTo compare the determination methods of total flavonoids in ramie seeds. MethodsThe ultraviolet-visible spectra of 2 standard reference substance (rutin and quercetin) and total flavonoids (solvent: 70% ethanol) in ramie seeds based on the direct colorimetry, NaNO2-Al3+-NaOH method and AlCl3 method were compared, and the selected method was evaluated by precision, stability, interference test, and recovery test. ResultsBoth rutin and quercetin were not suitable as a standard reference substance for the direct determination of total flavonoids in ramie seeds. The maximum absorption wavelength was 500 nm for total flavonoids by NaNO2-Al3+-NaOH method. However the total flavonoid content measured was higher using NaNO2-Al3+-NaOH method due to interference with certain non-flavonoids. For the AlCl3 method, the measurement wavelength was 401 nm using rutin as a reference substance, and this method had a good linear relationship in the concentration range of 0.004?0.02 mg/mL (r2=0.9998). The AlCl3 method had the advantages of low interference and high precision. The average recovery rate was (99.46±4.16)%. The absorbance was stable within 20?60 min after color development. ConclusionThe AlCl3 method is simple, feasible, and is suitable for the determination of total flavonoids in ramie seeds extract.
标题：苎麻籽总黄酮含量测定方法比较研究
英文标题：Comparative study on determination method of total flavonoids contentin ramie seeds
作者：
赵振 湖南农业大学食品科学技术学院,食品科学与生物技术湖南省重点实验室
刘霞 湖南农业大学食品科学技术学院,食品科学与生物技术湖南省重点实验室
黄昭 湖南农业大学食品科学技术学院,食品科学与生物技术湖南省重点实验室
李跑 湖南农业大学食品科学技术学院,食品科学与生物技术湖南省重点实验室
邢虎成 湖南农业大学苎麻研究所;湖南省种质资源创新与资源利用重点实验室
曹亚男 湖南农业大学食品科学技术学院,食品科学与生物技术湖南省重点实验室
中文关键词:苎麻籽,总黄酮,分光光度法,
英文关键词:ramie seeds,total flavonoids,spectrophotometry,
发表日期：2019-07-08
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