大豆转基因成分测定能力验证结果与分析
大豆转基因成分测定能力验证结果与分析
目的进一步提高实验室对转基因成分的检测水平, 提升检验人员的专业素养。方法根据能力验证计划作业指导书中推荐使用的GB/T 19495.5-2004《转基因产品检测 核酸定量PCR检测方法》中提供的3种方法分别对样品进行检测。结果样品013和083为GTS-40-3-2品系转基因大豆, 142为非转基因大豆。能力验证获得满意结果。结论转基因成分检测应重点关注DNA提取效率和实验过程质控等因素。
ObjectiveTo improve the detection ability of genetically modified component in laboratory, and enhance the technical level of laboratory technicians. MethodsThe detection of samples was carried out, according to the 3 kinds of methods provided in GB/T 19495.5-2004 Detection of genetically modified organisms and derived products-Quantitative PCR methods based on nucleic acid recommended in the capability test operation guide. ResultsThe foreign gene GTS-40-3-2 was detected in CODE 013 and CODE 083. Meanwhile, GTS-40-3-2 wasn’t detected in CODE 142. The ability verification results were satisfied by the organizers. ConclusionThe detection of genetically modified components should focus on factors such as DNA extraction efficiency and quality control of the experimental process.
标题：大豆转基因成分测定能力验证结果与分析
英文标题：Proficiency testing results and analysis of detection of genetically modified component in soybean
作者：
沙淼 西安市食品药品检验所
李亚楠 西安市食品药品检验所
李宏铎 西安市食品药品检验所
杨晓东 西安市食品药品检验所
中文关键词:大豆,转基因成分,能力验证,
英文关键词:soybeans,genetically modified component,proficiency testing,
发表日期：2019-01-24
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