TaqMan荧光PCR法快速检测太平洋鳕鱼源性成分
TaqMan荧光PCR法快速检测太平洋鳕鱼源性成分
目的建立TaqMan荧光PCR法检测太平洋鳕鱼源性成分的方法。方法以太平洋鳕鱼线粒体细胞色素氧化酶CoxⅠ检测靶标, 设计特异性引物和探针, 进行特异性和灵敏度试验, 并对市售样品进行检测。结果该方法引物探针特异性和灵敏度较好, 可以良好区分近属鳕鱼和其他科鱼类, 灵敏度达到0.04 ng/μL。市售样品中, 90.9%太平洋鳕鱼切片及71.4%太平洋鳕鱼罐头中检出太平洋鳕鱼成分。结论该方法的特异性和灵敏度较好, 适合用于大多数制品中太平洋鳕鱼成分的真实性甄别。
ObjectiveTo establish a method of rapid identification of the authenticity of Gadus macrocephalussource components by TaqMan fluorescent PCR. MethodsTargets were detected by Gadus macrocephalus mitochondrial cytochrome oxidase CoxI, specific primers and probes were designed, specificity and sensitivity tests were performed, and commercially available samples were tested. ResultThe primers of this method had good specificity and sensitivity, and could well distinguish between Gadus macrocephalus and other species of fish, with a sensitivity of 0.04 ng/μL.Among the commercially available samples, Gadus macrocephalus were found in 90.9% of Gadus macrocephalus slices and 71.4% of Gadus macrocephalus canned food. ConclusionThe method has good specificity and sensitivity, which is suitable for the authenticity screening of Gadus macrocephalus componentsin most products.
标题：TaqMan荧光PCR法快速检测太平洋鳕鱼源性成分
英文标题：Rapid identification of Gadus macrocephalus source componentsby TaqMan fluorescent PCR
作者：
万超 大连海关技术中心
王雷 大连海关技术中心
王贵滨 大连海关技术中心
杨宇 大连海关技术中心
中文关键词:CoxⅠ,荧光PCR,太平洋鳕鱼,
英文关键词:CoxⅠ,TaqMan real-time fluorescence PCR,Gadus macrocephalus,
发表日期：2018-12-11
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