米酵菌酸荧光定量检测卡的开发和应用
米酵菌酸荧光定量检测卡的开发和应用
目的开发一种快速定量检测米面等食品中米酵菌酸的方法。方法首先, 制备米酵菌酸-牛血清白蛋白偶联物(bongkrekic acid-bovine serum albumin, BA-BSA), 作为检测卡的包被原料包被至硝酸纤维素膜上, 用本公司自制BA单克隆抗体与时间分辨微球标记放入微孔中, 然后将包被后的膜和标记后的微孔组装成快速检测卡。结果快速检测卡的线性范围为1~100 ng/mL, 前处理采用碱性甲醇水溶液提取样本中米酵菌酸。结论本方法快速、准确、灵敏度高, 适合样品中米酵菌酸的检测。
ObjectiveTo develop a method for rapid and quantitative detection of bongkrekic acid in foods that made of rice and wheat. MethodsBongkrekic acid-bovine serum albumin conjugate (BA-BSA)was synthetized and coated on the nitrocellulose membrane at first, and then BA monoclonal antibody produced in Eugene was labeled by TRF microspheres and was freeze-dried in microwells. Membrane and microwell that prepared before were assembled into test kits. ResultsThe BA test kits had a wide detection range (1-100 ng/mL), and the tris-methanol reagent could be easily used for extracting BA from food samples. ConclusionThis method is rapid, accurate and sensitive, which is suitable for detecting bongkrekic acid in feed samples.
标题：米酵菌酸荧光定量检测卡的开发和应用
英文标题：Development and application of fluorescence quantitative detection card for bongkrekic acid
作者：
张小波 上海优晶生物科技有限公司
辛苗苗 上海优晶生物科技有限公司
温国源 上海优晶生物科技有限公司
郑嘉庚 上海优晶生物科技有限公司
李峰 上海优晶生物科技有限公司
中文关键词:米酵菌酸,时间分辨荧光,快速检测,免疫层析技术,
英文关键词:bongkrekic acid,time-resolved fluorescence,rapid test,immunochromatography,
发表日期：2019-04-15
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