基于DNA保护的银纳米簇荧光探针检测啶虫脒
基于DNA保护的银纳米簇荧光探针检测啶虫脒
目的设计合成DNA-AgNCs荧光探针用于检测啶虫脒。方法以啶虫脒的适配体DNA为保护剂, 硼氢化钠为还原剂还原AgNO3, 合成发射波长为421 nm的DNA-AgNCs。啶虫脒溶液与该纳米簇混合时, 荧光强度降低, 根据该现象检测啶虫脒。结果在37 ℃的条件下, 啶虫脒于DNA-AgNCs体系中(在pH=7.0的磷酸缓冲溶液中)反应60 min后荧光猝灭, 啶虫脒的浓度在5~200 μmol/L范围内呈现出良好的线性关系, 检出限为2.8 μmol/L(S/N=3)。结论该检测方法较简单, 易操作, 快速、准确、灵敏, 适用于测定啶虫脒。
ObjectiveTo design a synthetic DNA-AgNCs fluorescent probe for the detection of acetamiprid. MethodThe aptamer DNA of acetamiprid was used as a template, and NaBH4 was used as reducing agent to get the DNA-AgNCs with an emission wavelength of 421 nm. When acetamiprid solution was mixed with the nanoclusters, the fluorescence intensity was reduced, and acetamiprid was detected according to this phenomenon. ResultsAt 37 ℃, the fluorescence of acetamiprid in the DNA-AgNCs system (in phosphate buffer at pH=7.0) was quenched after 60 min. Acetamiprid had good linear relationship between the fluorescent intensity and the acetamiprid concentration in the range of 5-200 μmol/L, with the limit of detection of 2.8 μmol/L (S/N=3). ConclusionThis method is simple, easy to operate, accurate and sensitive, which is suitable for detection of acetamiprid.
标题：基于DNA保护的银纳米簇荧光探针检测啶虫脒
英文标题：Sensitive detection of acetamiprid silver nanoclusters fluorescent probes based on DNA protection
作者：
边孟孟 桂林理工大学化学与生物工程学院
李小青 桂林理工大学化学与生物工程学院
袁亚利 桂林理工大学化学与生物工程学院
中文关键词:荧光检测,银纳米簇,啶虫脒,
英文关键词:fluorescent detection,silver nanoclusters,acetamiprid,
发表日期：2019-04-01
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