高效液相色谱法测定保健食品中腺苷的含量
高效液相色谱法测定保健食品中腺苷的含量
目的建立反相高效液相色谱法(high performance liquid chromatography, HPLC)测定保健食品中功效成分腺苷。方法样品中腺苷经85%的甲醇溶液加热回流提取, C18反相柱分离, 在波长260 nm处进样检测。结果在5.045~252.250 μg/mL范围内具有良好的线性关系, 回归方程是Y=27281X?57639, 相关系数r=0.9999, 检出限为0.5 μg/mL(S/N=3), 此方法的回收率为91.5%~97.7%, 相对标准偏差(relative standard deviation, RSD)为1.5%~2.8%。结论此方法简便快速, 准确可靠, 可用于各类保健食品中腺苷含量的测定。
ObjectiveTo establish a method for determination of adenosine of functional ingredients in health food by reversed phase-high performance liquid chromatography (HPLC). MethodThe adenosine from sample was heating refluxed by 85% methanol, and then separated by C18 column and detected at the wavelength of 260 nm. ResultsThere was a good linear relationship in the range of 5.045-252.250 μg/mL. The regression equation was Y=27281X?57639, with the correlation coefficient (r) of 0.9999, and the limit of detection was 0.5 μg/mL (S/N=3). The recoveries were 91.5%-97.7%, and relative standard deviations (RSDs) were 1.5%-2.8%. ConclusionThis method is simple, rapid, accurate and reliable, which can be used for the determination of adenosine content in all kinds of health food.
标题：高效液相色谱法测定保健食品中腺苷的含量
英文标题：Determination of adenosine in health food by high performance liquid chromatography
作者：
孙艳芳 黑龙江省疾病预防控制中心
张剑峰 黑龙江省疾病预防控制中心
张春玲 黑龙江省疾病预防控制中心
王缘 黑龙江省疾病预防控制中心
孙海会 黑龙江省疾病预防控制中心
中文关键词:腺苷,高效液相色谱法,保健食品,,
英文关键词:Adenosine,high performance liquid chromatography,health food,,
发表日期：2018-02-02
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