超高效液相色谱-串联质谱法测定畜禽肉中17种磺胺类药物残留
超高效液相色谱-串联质谱法测定畜禽肉中17种磺胺类药物残留
目的建立超高效液相色谱-串联质谱(ultra performance liquid chromatography-tandem mass spectrometry, UPLC-MS/MS)测定畜禽肉组织中17种磺胺类药物残留的方法。方法样品经乙腈提取、浓缩, 正己烷脱脂后在正离子模式下以电喷雾电离串联质谱进行测定, 外标法定量。结果17 种磺胺类药物在 5 μg/L~100 μg/L范围内线性良好, 相关系数＞0.99, 方法检出限为2 μg/kg, 定量限为5 μg/kg。在10、20、 100 μg/kg的添加水平下, 17种磺胺类药物的平均回收率在84.0%~114.2%之间, 相对标准偏差(relative standard deviation, RSD, n=6)在0.59%~7.96%之间。结论本方法快速、准确、灵敏, 可应用于畜禽肉中17种磺胺类药物残留的同时检测。
ObjectiveTo establish an analytical method for determination of 17 kinds of sulfonamide residues in livestock and poultry meat by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). MethodsThe samples were extracted and enriched by acetonitrile and determined by multiple reaction monitoring (MRM) mode with electrospray ionization source, then quantified by external standard method. ResultsAll of the analytes showed good linearity in the range of 5 μg/L~100 μg/kg and the correlation coefficients (r2) were all above 0.99. The limits of detection (LODs) for the derivatized sulfonamide residues were 2 μg/kg and the limits of quantification (LOQs) were 5 μg/kg. The average recoveries for the blank samples at 3 spiked levels (10, 20, 100 μg/kg) ranged from 84.0% to 114.2%, with the relative standard deviations (RSDs, n=6) of 0.59%~7.96%. ConclusionThe proposed method is rapid, accurate and sensitive, which is suitable for simultaneous detecting of 17 kinds of sulfonamide residues in livestock.
标题：超高效液相色谱-串联质谱法测定畜禽肉中17种磺胺类药物残留
英文标题：Determination of 17 kinds of sulfonamide residues in livestock and poultry meat by ultra performance liquid chromatography-tandem mass spectrometry
作者：
任丹丹 中粮营养健康研究院,营养健康与食品安全北京市重点实验室
谢云峰 中粮营养健康研究院,营养健康与食品安全北京市重点实验室
李少晖 中粮营养健康研究院,营养健康与食品安全北京市重点实验室
杨永坛 中粮营养健康研究院,营养健康与食品安全北京市重点实验室
中文关键词:磺胺类,超高效液相色谱-串联质谱法,畜禽肉,
英文关键词:sulfonamide,ultra performance liquid chromatography-tandem mass spectrometry,livestock and poultry meat,
发表日期：2017-12-04
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