高效液相色谱法测定运动饮料中13种食品添加剂
高效液相色谱法测定运动饮料中13种食品添加剂
目的建立高效液相色谱法(high performance liquid chromatography, HPLC)同时快速测定运动饮料中13种食品添加剂的检测方法。方法在40 ℃柱温, 1.0 mL/min流速下, 选取phenomenex C18(250 mm× 4.6 mm, 5 μm) 色谱柱, 在0.02 mol/L乙酸铵(pH 6.86)和甲醇为流动相条件下进行梯度洗脱, 采用二极管阵列检测器在230 nm波长处进行检测, 外标法定量, 实现同时对13种食品添加剂的分离。结果该方法线性良好, 相关系数均大于0.990, 检出限为0.2~0.5 mg/kg, 平均回收率为81.0%~107.7%, 相对标准偏差(relative standard deviations, RSDs)为1.00%~5.49%。结论建立的高效液相色谱法满足同时检测运动饮料中13种食品添加剂的要求, 操作简单、灵敏度高、重复性好。
ObjectiveTo establish a method for simultaneous determination of 13 kinds of food additives in sports drinks by high performance liquid chromatography (HPLC). MethodsThe separation was achieved on a phenomenex C18 column (250 mm×4.6 mm, 5 μm) by using 0.02 mol/L NH4Ac(pH 6.86) and methyl alcohol as mobile phase with a flow rate of 1.0 mL/min at 40 ℃. A diode array detector was used to detect at 230 nm. The quantification was performed by the external standard method．ResultsThe method showed good linearities with correlation coefficients (r2) more than 0.990. The limits of detection were 0.2~0.5 mg/kg. The average recoveries of 13 kinds of food additives were 81.0%~107.7% with relative standard deviations (RSDs) of 1.00%~ 5.49%．ConclusionThe established method is simple, rapid, and accurate, which is suitable for quantitative analysis of 13 kinds of food additives in sports drinks.
标题：高效液相色谱法测定运动饮料中13种食品添加剂
英文标题：Simultaneous determination of 13 kinds of food additives in sports drinks by high performance liquid chromatography
作者：
刘仲义 广东产品质量监督检验研究院
李锦清 广东产品质量监督检验研究院
伍尚森 广东产品质量监督检验研究院
伍志航 广东产品质量监督检验研究院
李聪 广东产品质量监督检验研究院
中文关键词:食品添加剂,高效液相色谱法,运动饮料,
英文关键词:food additive,high performance liquid chromatography,sports drink,
发表日期：2017-10-15
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