在线柱前衍生高效液相色谱法测定古树红茶中的γ-氨基丁酸
在线柱前衍生高效液相色谱法测定古树红茶中的γ-氨基丁酸
目的建立在线柱前衍生高效液相色谱法(high performance liquid chromatography, HPLC)测定古树红茶中的γ-氨基丁酸的方法。方法样品在85 ℃水浴中浸提2 h, 石墨化炭黑(graphitizing of carbon black, GCB)净化, 经自动在线柱前衍生后, 用Agilent ZORBAX SB-C18 (250 mm×4.6 mm, 5 μm)色谱柱分离, 用紫外检测器于338 nm波长处进行测定。结果γ-氨基丁酸在2~80 μg/mL范围内线性关系良好(r=0.9994), 回归方程为Y=10.224X?17.534, 加标平均回收率为95.2%~96.8%, 相对标准偏差为1.7%~2.9% (n=3)。结论此方法具有良好的精密度、稳定性及回收率, 可用于古树红茶中的γ-氨基丁酸测定。
ObjectiveTo establish the method for determination of γ-aminobutyric acid in ancient tree black tea by high performance liquid chromatography (HPLC) with online derivatization. MethodsSamples were extracted in 85 ℃ water for 2 h and refined by graphitizing of carbon black (GCB). After derivated by automatic online column and separated by Agilent ZORBAX SB-C18 (250 mm×4.6 mm, 5 μm), the samples were measured by ultraviolet absorption detector at 338 nm. Resultsγ-aminobutyric acid had a good linearity in the range of 2-80 μg/mL with a correlation coefficient of 0.9994, the regression equation was Y=10.224X?17.534, the recoveries of formaldehyde were in the range of 95.2%-96.8%, and the relative standard deviations were in the range of 1.7%-2.9% (n=3). ConclusionThis method has the advantages of good precision, stability and high recovery, which is suitable for the determination of γ-aminobutyric acid in ancient tree black tea.
标题：在线柱前衍生高效液相色谱法测定古树红茶中的γ-氨基丁酸
英文标题：Determination of γ-aminobutyric acid in ancient tree black tea by high performance liquid chromatography with online pre-column derivatization
作者：
段静 中国食品药品检定研究院
李莉 中国食品药品检定研究院
李硕 中国食品药品检定研究院
王海燕 中国食品药品检定研究院
曹进 中国食品药品检定研究院
丁宏 中国食品药品检定研究院
中文关键词:高效液相色谱法,在线衍生,γ-氨基丁酸,红茶,
英文关键词:high performance liquid chromatography,online derivatization,γ-aminobutyric acid,black tea,
发表日期：2018-07-10
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